Fig. 3

Inhibitors of endosomal acidification suppressed SARS-CoV-2 replication in hACE2 transgenic mice. hACE2 transgenic mice were inoculated with 6.7 × 10³ PFU of SARS-CoV-2 intratracheally in 30 μl of PBS. PBS, chloroquine (60 mg kg⁻¹ once daily), or bafilomycin A1 (0.1 mg kg⁻¹ once daily), were then given intraperitoneally for 5 days. Organ tissues were collected on day 5. a Viral genome copies in lung tissues. b, c In situ hybridization for SARS-CoV-2 RNA in lung tissues. d–f Viral genome copies in primary organs. g Body weight changes. Three experiments were performed (n = 6 each group). **p < 0.01, ***p < 0.001. Green bar = 25 μm. Red bar = 50 μm