Fig. 2

Inhibitors of endosomal acidification suppressed SARS-CoV-2 replication in vitro. Cells were infected with SARS-CoV-2 (0.008 MOI) and cell supernatant was harvested on indicated time points post infection. SARS-CoV-2 (0.008 MOI) was diluted in cell supernatant and harvested immediately as samples of 0 h post infection. Viral genome copies in Vero E6 cells (a), Huh-7 (b), and 293T-ACE2 cells (c) treated with PBS, chloroquine (40 μM), bafilomycin A1 (100 nM) or NH₄CL (12.5 mM). Three experiments were performed (n = 3 each group). *p < 0.05, **p < 0.01, ***p < 0.001