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Fig. 1 | Virology Journal

Fig. 1

From: HIV-1 Vpr activates host CRL4-DCAF1 E3 ligase to degrade histone deacetylase SIRT7

Fig. 1

SIRT7 binds to the substrate receptor/adaptor complex, DCAF1/DDB1 of CRL4. a Control plasmids or plasmids expressing DCAF1 or SIRT7 were transiently co-transfected into HEK293T cells as indicated. 48 h later, cells were harvested and cell lysates were prepared for immunoprecipitation (IP) with anti-FLAG antibody. Both lysate and IP elution fractions were subjected to SDS-PAGE and immunoblotting with appropriate antibodies. b Control plasmids or plasmids expressing SIRT7, DCAF1 or DDB1 were transiently co-transfected into HEK293T cells as indicated. Cells were treated as in a. Endogenous DDB1 is indicated (*). c The mixture of plasmids expressing DDB1, DCAF1, and Vpr were co-transfected into HEK293T cells with or without plasmids expressing SIRT7. Immunoprecipitation of SIRT7 was performed with anti-FLAG antibody. d Plasmids expressing Vpr were co-transfected with plasmids expressing SIRT7 or control plasmids into HEK293T cells. SIRT7 proteins were immunoprecipitated with anti-FLAG antibody and probed with immunoblotting. Experiments were repeated with two to three times with similar results

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