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Table 2 Summary of upregulated proteins in A549 cells infected with influenza A H5N1 virus compared to H9N2-infected cells at 24 hpi (r > 2, p < 0.05)

From: Differential mitochondrial proteomic analysis of A549 cells infected with avian influenza virus subtypes H5 and H9

Spot no.a Accession No.b GENE Protein name MW (Da) pI Scorec
17* P30084 ECHS1 Enoyl-CoA hydratase, mitochondrial 31,716 6.05 68
18 P02768 ALB Serum albumin 47,098 5.92 64
19 P35527 KRT9 Keratin, type I cytoskeletal 9 62,255 5.14 72
20 Q9HC85 MB2 Metastasis related protein 10,414 5.16 35
21 P11021 HSPA5 Endoplasmic reticulum chaperone BiP 71,002 5.23 155
22 AIZ70879 N/A immunoglobulin heavy chain variable region, partial 8328 11.84 42
23* P34931 HSPA1L heat shock 70-kDa protein 1-like 77,913 7.55 64
24 A0A1L1UHR1 HTL-T-186e Homo sapiens sperm binding protein 1aing mRNA 30,868 8.62 46
25 P09651 HNRNPA1 Heterogeneous nuclear ribonucleoprotein A1 34,289 9.27 58
26 P22626 HNRNPA2B1 Heterogeneous nuclear ribonucleoproteins A2/B1 36,041 8.67 52
27 P08670 VIM vimentin isoform 1, partial 53,676 5.06 56
28 Q8N1N4 KRT78 Keratin, type II cytoskeletal 78 57,728 5.79 51
29 Q86Y46 KRT73 Keratin, type II cytoskeletal 73 42,270 8.42 51
30 D9YZU9 HBG2 TPA: globin B2 16,173 6.64 50
31 P68871 HBB Beta globin 11,534 5.9 46
32 P69905 HBA1 Hemoglobin subunit alpha 15,174 8.73 65
  1. aSpot No. is the unique sample spot protein number
  2. bAccession No is the MASCOT result of MALDI-TOF/TOF searched from the NCBI database
  3. cProtein score (based on combined MS and MS/MS spectra) and best ion score (based on MS/MS spectra) were from MALDI-TOF/TOF identification
  4. *Indicating that the differential proteins were validated by western blot analysis
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Virology Journal

ISSN: 1743-422X