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Table 4 Comparison multiplex real-time PCR with DNA sequencing

From: A multiplex real-time PCR quantitation of human herpesvirus-6, 7, 8 viruses: application in blood transfusions

Subject No. (%) positive numbers
  HHV-6 HHV-7 HHV-8 HHV-6&HHV-7
Multiplex PCR (blood donors) 12 (16.2) 41 (55.4) 0 (0) 8 (10.8)
DNA sequencing (blood donors) 11 (14.8) 38 (51.3) 1 (1.3) 6 (8.1)
Sensitivity (blooddonors) 100% 100% 0% 100%
Specificity (blood donors) 98.4% 91.8% 100% 97.1%
Multiplex PCR (clinical samples) 18 (40) 33 (73.3) 1 (2.2) 13 (28.8)
DNA sequencing (clinical samples) 16 (35.5) 30 (66.7) 1 (2.2) 10 (22.2)
Sensitivity (clinical samples) 100% 100% 100% 100%
Specificity (clinical samples) 93.1% 80% 100% 91.4%