Table 4 Comparison multiplex real-time PCR with DNA sequencing
Subject | No. (%) positive numbers | |||
|---|---|---|---|---|
HHV-6 | HHV-7 | HHV-8 | HHV-6&HHV-7 | |
Multiplex PCR (blood donors) | 12 (16.2) | 41 (55.4) | 0 (0) | 8 (10.8) |
DNA sequencing (blood donors) | 11 (14.8) | 38 (51.3) | 1 (1.3) | 6 (8.1) |
Sensitivity (blooddonors) | 100% | 100% | 0% | 100% |
Specificity (blood donors) | 98.4% | 91.8% | 100% | 97.1% |
Multiplex PCR (clinical samples) | 18 (40) | 33 (73.3) | 1 (2.2) | 13 (28.8) |
DNA sequencing (clinical samples) | 16 (35.5) | 30 (66.7) | 1 (2.2) | 10 (22.2) |
Sensitivity (clinical samples) | 100% | 100% | 100% | 100% |
Specificity (clinical samples) | 93.1% | 80% | 100% | 91.4% |