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Table 2 Optimization of PCR conditions

From: A multiplex real-time PCR quantitation of human herpesvirus-6, 7, 8 viruses: application in blood transfusions

Type of ingredient Gradient (variation rate) Optimal ultimate concentration (type of virus)
Mgcl2a 2.5 mM–5.5 mM (0.5) 3.5 mM (HHV-6) 4 mM (HHV-7) 3.5 mM (HHV-8)
Deoxynucleotide mixturea 0.14 mM–0.32 mM (0.02) 0.16 mM (HHV-6) 0.16 mM (HHV-7) 0.16 mM (HHV-8)
Primera 0.26 μM–0.35 μM (0.01) 0.29 μM (HHV-6) 0.29 μM (HHV-7) 0.31 μM (HHV-8)
Probea 0.12 μM–0.40 (0.02) 0.24 μM (HHV-6) 0.26 μM (HHV-7) 0.24 μM (HHV-8)
Taq DNA polymerasea,b 0.1U–0.6U (0.1) 0.5U (HHV-6) 0.6U (HHV-7) 0.6U (HHV-8)
  1. aInitial concentration of Mgcl2, deoxynucleotide mixturea, primer, probe, Taq DNA Polymerase is 25 mM, 10 mM, 10 μM, 10 μM, 5U/μL respectively
  2. bTaq DNA Polymerase was obtained from TaKaRa Biotechnology Dalian Co. Ltd., Liaoning, China