Table 1 The oligonucleotide sequences of primers and TaqMan-LNA probes sequences
Virus type | Target gene (NOa) | Sequence (5′ → 3′) (forward and reverse primer, probe) | Positionsb (position of HHV-6B) | Sizec (bp) | Fluorophore label |
|---|---|---|---|---|---|
HHV-6 | U67(NC_001664d) | TAAATATCGATGCCGCTCTG | 102,708–102,727 (104,006–104,025) | 76 | 6FAM-BHQ-2 |
(NC_000898e) | TACGTTCTAGCCATCTTCTTTG | 102,762–102,783 (104,060–104,081) | |||
CGCAAACGACAAAGCCA | 102,735–102,751 (104,033–104,049) | ||||
HHV-7 | U36(NC_001716) | TTAGACATCTTACACGACAGC | 55,407–55,427 | 147 | JOE-BHQ-2 |
CAGCTTTTCGAACTTGTCAC | 55,534–55,553 | ||||
TTCATCGGGTACGTCCA | 55,513–55,529 | ||||
HHV-8 | ORF65(AF148805) | GCGACATATTTCCCTGATCC | 112,438–112,457 | 108 | Cy5-BHQ-2 |
CCAACTTTAAGGTGAGAGACC | 112,525–112,545 | ||||
CATGCGAGCCACCAGG | 112,466–112,481 |