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Fig. 3 | Virology Journal

Fig. 3

From: Immunostimulatory siRNA with a uridine bulge leads to potent inhibition of HBV and activation of innate immunity

Fig. 3

Inhibitory effects of msiHBx on HBV replication and gene expression. Huh7 cells were cotransfected with 1 µg of pHY106-wta and 20 nM msiHBx, siHBx, or siNC as a control. The levels of HBsAg and HBeAg in cell culture supernatants (a), HBV replicative intermediates (b, e), HBV DNA in cell culture supernatants (d), and HBV RNA in cells (c) were analyzed at 72 h after transfection by ELISA, Southern blotting, PCR, and One-Step RT-PCR, respectively. HepG2.2.15 cells were transfected with 20 nM msiHBx, siHBx, or siNC as a control. The HBsAg and HBeAg levels in cell culture supernatants (f) and HBV replicative intermediates (g, h) were analyzed at 72 h after transfection by ELISA, Southern blotting, and PCR, respectively. ns, no significant difference. *p < 0.05. **p < 0.01. ***p < 0.001. siNC was purchased from Sangon Biotech (Shanghai, China) and used as a negative control. siHBx, siRNA targeting the HBV X gene. msiHBx, msiRNA targeting the HBV X gene. RC, relaxed circular DNA. DL, double-stranded linear DNA. SS, single-stranded DNA

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