Fig. 1

The sequences and structures of siRNAs and msiRNAs with unpaired uracil bulges. The uracil substitution at the 9–12th nucleotides of the passenger strand of siRNAs is indicated in red. siHBs, siRNA targeting the HBV S gene. siHBx, siRNA targeting the HBV X gene. msiHBs, msiRNA targeting the HBV S gene. msiHBx, msiRNA targeting the HBV X gene. siNC was purchased from Sangon Biotech (Shanghai, China) and used as a negative control