Target genes
|
Primers
|
Primer sequence (5′–3′)
|
Location (nt)a
|
Annealing temp (°C)
|
---|
N
|
N(F)
|
GCGAAAAAAACGCGTATA
|
3–20
|
50
|
|
N(R)
|
TTGCTGCTTCATTACCCAT
|
1296–1314
| |
P
|
P(F)
|
GAAGAAAAAGARGCTGCAGA
|
1178–1196
|
50
|
|
P(R)
|
CCAGATCAACTTGAACAGC
|
2228–2246
| |
M
|
M(F)
|
GAATCMGAAGAAGAAGAAG
|
2070–2088
|
50
|
|
M(R)
|
CAYCACTTTCCAAGACATT
|
3066–3084
| |
F
|
F-1(F)
|
CAATCMAAAAGGYATATTC
|
2800–2818
|
50.2
|
|
F-1(R)
|
CTGCAGATGTYGGCATGT
|
3764–3781
| |
|
F-2(F)
|
CAGTCAATTCAACAGAAG
|
3645–3662
|
50
|
|
F-2(R)
|
TTCATATTTGCATGGAGC
|
4736–4753
| |
M2
|
M2(F)
|
CCTCCAGAGCTGARTGGTGT
|
4636–4655
|
50
|
|
M2(R)
|
TWTCATTGTCAYTTATCCCA
|
5467–5486
| |
SH
|
SH(F)
|
GCAAGACAGTGAAAGCAYT
|
5254–5272
|
46.8
|
|
SH(R)
|
CATCYTTGCTTTGARCAT
|
6268–6285
| |
G
|
G(F)
|
AAACAARAAWATGGGACAAG
|
6207–6226
|
50
|
|
G(R)
|
TAGACATTRACAGTRGAYTC
|
7148–7167
| |
L
|
L-1(F)
|
GACAAATRRCAATGGATC
|
7122–7139
|
44.7
|
|
L-1(R)
|
TAGTTCTGTYAARCTCTC
|
8303–8320
| |
|
L-2(F)
|
GARAATGCTGCWGAATTA
|
8186–8203
|
46.8
|
|
L-2(R)
|
GATATHGCYTCCATTGTCC
|
9288–9306
| |
|
L-3(F)
|
ACATGCACCACCAGAAAC
|
9178–9195
|
46.8
|
|
L-3(R)
|
AGGTGTTATGTTKTCWGC
|
10,238–10,225
| |
|
L-4(F)
|
GTGAYATMAATAGAACAGC
|
10,128–10,146
|
43.8
|
|
L-4(R)
|
ACTGAAGATRTGTTGATC
|
11,231–11,248
| |
|
L-5(F)
|
GGAATTAGYATAATGAGTG
|
11,081–11,099
|
48
|
|
L-5(R)
|
GGATATTCACATGCTGTTC
|
12,246–12,264
| |
|
L-6(F)
|
CTAACAAGRAATTACATG
|
12,071–12,088
|
48
|
|
L-6(R)
|
ACGGCAAAAAAACCGTAT
|
13,318–13,335
| |
- aThe reference of location is based on AY297749 strain