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Table 1 Primer sets for amplification of the whole genome of HMPV

From: Epidemiology and genotypic diversity of human metapneumovirus in paediatric patients with acute respiratory infection in Beijing, China

Target genes

Primers

Primer sequence (5′–3′)

Location (nt)a

Annealing temp (°C)

N

N(F)

GCGAAAAAAACGCGTATA

3–20

50

 

N(R)

TTGCTGCTTCATTACCCAT

1296–1314

 

P

P(F)

GAAGAAAAAGARGCTGCAGA

1178–1196

50

 

P(R)

CCAGATCAACTTGAACAGC

2228–2246

 

M

M(F)

GAATCMGAAGAAGAAGAAG

2070–2088

50

 

M(R)

CAYCACTTTCCAAGACATT

3066–3084

 

F

F-1(F)

CAATCMAAAAGGYATATTC

2800–2818

50.2

 

F-1(R)

CTGCAGATGTYGGCATGT

3764–3781

 
 

F-2(F)

CAGTCAATTCAACAGAAG

3645–3662

50

 

F-2(R)

TTCATATTTGCATGGAGC

4736–4753

 

M2

M2(F)

CCTCCAGAGCTGARTGGTGT

4636–4655

50

 

M2(R)

TWTCATTGTCAYTTATCCCA

5467–5486

 

SH

SH(F)

GCAAGACAGTGAAAGCAYT

5254–5272

46.8

 

SH(R)

CATCYTTGCTTTGARCAT

6268–6285

 

G

G(F)

AAACAARAAWATGGGACAAG

6207–6226

50

 

G(R)

TAGACATTRACAGTRGAYTC

7148–7167

 

L

L-1(F)

GACAAATRRCAATGGATC

7122–7139

44.7

 

L-1(R)

TAGTTCTGTYAARCTCTC

8303–8320

 
 

L-2(F)

GARAATGCTGCWGAATTA

8186–8203

46.8

 

L-2(R)

GATATHGCYTCCATTGTCC

9288–9306

 
 

L-3(F)

ACATGCACCACCAGAAAC

9178–9195

46.8

 

L-3(R)

AGGTGTTATGTTKTCWGC

10,238–10,225

 
 

L-4(F)

GTGAYATMAATAGAACAGC

10,128–10,146

43.8

 

L-4(R)

ACTGAAGATRTGTTGATC

11,231–11,248

 
 

L-5(F)

GGAATTAGYATAATGAGTG

11,081–11,099

48

 

L-5(R)

GGATATTCACATGCTGTTC

12,246–12,264

 
 

L-6(F)

CTAACAAGRAATTACATG

12,071–12,088

48

 

L-6(R)

ACGGCAAAAAAACCGTAT

13,318–13,335

 
  1. aThe reference of location is based on AY297749 strain