Skip to main content
Fig. 2 | Virology Journal

Fig. 2

From: Development of a reverse transcription droplet digital PCR (RT-ddPCR) assay for sensitive detection of simian immunodeficiency virus (SIV)

Fig. 2

Two step RT-ddPCR test. The following condition combinations were tested during the reverse transcription step of RT-ddPCR: M-MLV RT with gene specific priming (a background; b SIV RNA standard spike); SSIII RT with gene specific priming (c background; d SIV RNA standard spike); M-MLV RT with random hexamer priming (e background; f SIV RNA standard spike); SSIII RT with random hexamer priming (g background; h SIV RNA standard spike). Each reaction contained 1 μg Rhesus macaque RNA background. The ddPCR step of all reactions was performed with SIV and CCR5 duplex MGB probe assays. Detailed experimental conditions are listed in Table 1

Back to article page
\