Fig. 2From: Development of a reverse transcription droplet digital PCR (RT-ddPCR) assay for sensitive detection of simian immunodeficiency virus (SIV)Two step RT-ddPCR test. The following condition combinations were tested during the reverse transcription step of RT-ddPCR: M-MLV RT with gene specific priming (a background; b SIV RNA standard spike); SSIII RT with gene specific priming (c background; d SIV RNA standard spike); M-MLV RT with random hexamer priming (e background; f SIV RNA standard spike); SSIII RT with random hexamer priming (g background; h SIV RNA standard spike). Each reaction contained 1 μg Rhesus macaque RNA background. The ddPCR step of all reactions was performed with SIV and CCR5 duplex MGB probe assays. Detailed experimental conditions are listed in Table 1Back to article page