Fig. 1

One step RT-ddPCR test. One step RT-ddPCR was performed as described in “Materials and Methods” using the SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase. Assay/sample combinations tested were: SIV single plex assay with buffer (a) or SIV RNA standard spike (b), SIV single plex assay with 1 μg Rhesus macaque RNA background (c) or SIV RNA standard spiked in 1 μg Rhesus macaque RNA background (d), SIV and CCR5 duplex assay with buffer (e) or SIV RNA standard spike (f), and SIV and CCR5 duplex assay with 1 μg Rhesus macaque RNA background (g) or with SIV RNA standard spiked in 1 μg Rhesus macaque RNA background (h). Detailed experimental conditions are listed in Table 1. Note that in all panels there were background signals in putative SIV target signal region. Quantitation was not done due to background signals