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Fig. 5 | Virology Journal

Fig. 5

From: Combination gene therapy for HIV using a conditional suicidal gene with CCR5 knockout

Fig. 5

Characterization of H7 cell line for resistance to HIV infection by CCR5 tropic HIV a TZM-TKSR39 or H7 cells were infected with HIV reporter virus particles pseudotyped with Lai or YU-2 Envelope. Infection was determined 24 h post infection by measuring luciferase activity in the cultures. b DNA was isolated from TZM-TKSR39 cell line or H7 clone and site of CRISPR mediated CCR5 disruption amplified using specific primers. PCR product was digested using the T7EI and bands were resolved on an agarose gel. c TZM, TZM-TK-SR39 or H7 cells were stained for CD4, CXCR4 and CCR5 expression using specific antibodies and analyzed by flow cytometry

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