Fig. 3
From: Combination gene therapy for HIV using a conditional suicidal gene with CCR5 knockout
Dual transduction of TZM cells with TK-SR39 vector followed by CCR5gRNA-CRISPR/Cas9 Tat vector results in GFP expression and CCR5 KO. a Strategy for dual transduction of TZM cells with integrating lentiviral vector 1 to deliver Tat dependent TK-SR39 gene and with non-integrating lentiviral vector 2 to deliver Tat and CCR5gRNA-CRISPR/Cas9. GFP expression and CCR5 KO was determined over a period of time. b TZM cells were transduced with vector 1 followed by vector 2 as in part A. Down regulation of surface CCR5 expression was monitored over a period of time by flow cytometry