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Fig. 2 | Virology Journal

Fig. 2

From: Combination gene therapy for HIV using a conditional suicidal gene with CCR5 knockout

Fig. 2

Characterization of Tat expression and CCR5 KO via vector 2. a TZM cells were transfected with CCR5gRNA-CRISPR/Cas9 Tat vector, Control-gRNA-CRISPR/Cas9 Tat vector or pCDNA3.1 empty vector. Luciferase activity was determined 48 h post transfection. b TZM-TKSR39 cells were transfected as in part A above. GFP expression was measured 48 h post transfection via flow cytometry. c TZM cells were transfected as in part A above. Six days post transfection, cells were analyzed for CCR5 expression by flow cytometry. d DNA was isolated from control TZM cells or TZM cells transfected with the CCR5gRNA-CRISPR/Cas9 Tat vector. Genetic disruption of the CCR5 locus was determined by PCR followed by T7EI digestion

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