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Fig. 1 | Virology Journal

Fig. 1

From: Combination gene therapy for HIV using a conditional suicidal gene with CCR5 knockout

Fig. 1

Strategy of combination gene therapy approach for HIV and plasmid maps. a Incorporating CCR5 KO along with conditional suicide gene to achieve HIV cure. Killing of HIV infected cells is achieved only in the presence of GCV providing an additional layer of safety for in vivo use. b Vector maps for the dual transduction strategy for HIV cure. Vector 1 incorporates TK-SR39 gene along with selection marker GFP downstream of an IRES. Vector 2 expresses the tat gene along with CCR5 gRNA-CRISPR/Cas9 cassette. c Strategy for packaging the vector 1 using an integrating lentivirus system and vector 2 using a non-integrating lentivirus resulting in permanent TK-SR39 integration, CCR5 KO and transient GFP expression for cell enrichment

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