Fig. 5
From: Identification and screening of host proteins interacting with ORFV-ORF047 protein
ORF047 interacts with PABPC1 or FLC and SERP1. 293 T cells were transfected with different plasmid groups(pcDNA-3.1–3×HA-ORF047and pcDNA-3.1–3×Flag plasmids or pcDNA-3.1–3×HA-ORF047 and pcDNA-3.1–3×Flag-PABPC4 plasmids or O pcDNA-3.1–3×HA- ORF047 and pcDNA-3.1–3×Flag-PABPC4 plasmids or pcDNA-3.1–3×HA-ORF047 and pcDNA-3.1–3×Flag-SEPR1 plasmids) for immunoprecipitation. Immunoprecipitation analysis was carried out 48 h later using anti-HA monoclonal antibodies and anti-Flag antibodies. A Western blot analysis cells lysis transfected with different plasmid groups with anti-HA monoclonal antibodies, the results showed pcDNA-3.1–3×HA-ORF047 protein expression in 293 T cells lysis using anti-HA monoclonal antibodies B Western blot analyzed immunoprecipitation with anti-Flag monoclonal antibodies and immunoblotting with anti-HA monoclonal antibodies. The results showed the interaction between ORF047 with the PABPC4 protein or SERP1 or FLC protein. C Western blot analyzed immunoprecipitation and immunoblotting with anti-Flag monoclonal antibodies