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Table 2 List of primers used for GAPDH PCR, nested PCR, real-time PCR, and multiplex PCR

From: Detection of human papillomavirus genotypes, herpes simplex, varicella zoster and cytomegalovirus in breast cancer patients

Assay

Name

Sequence (5′ → 3′)

GAPDH

GAPDH-F

ATGTTCGTCATGGGTGTGAA

(110 bp)

GAPDH-R

GGTGCTAAGCAGTTGGTGGT

MY09/11

MY09

CGTCC (A/C) A (A/G) (A/G) GGA (A/T) ACTGATC

(450 bp)

MY11

GC (A/C) CAGGG (A/T) CTATAA(C/T) AATGG

GP5 + /GP6 + 

GP5 + 

TTTGTTACTGTGGTAGATACTAC

(150 bp)

GP6 + 

AAAAATAAACTGTAAATCATATTC

HPV 18 E6

HPV 18 E6-F

CTG GGC ACT ATA GAG GCC AGT

(78 bp)

HPV 18 E6-R

GTG TTT CTC TGC GTC GTT GG

 

HPV 18 E6-P

[FAM] -TGCAACCGAGCACGACAGGAACGA—[TAMRA]

HPV 16 E7

HPV 16 E7-F

GAG GAG GAG GAT GAA ATA GAT GGT

(98 bp)

HPV 16 E7-R

AGC GTA GAG TCA CAC TTG CAA CA

 

HPV 16 E7-P

[FAM] -CTCTGTCCGGTTCTGCTTGTCCAGCT- [TAMRA]

HSV-1

HSV-1-F

GACTCTCCCACCGCCATCAG

(269 bp)

HSV-1-R

TGTCTTCGGGCGACTGGT

HSV-2

HSV-2-F

TATGCCTATCCCCGGTTGGA

(715 bp)

HSV-2-R

CGTGCCATCCGAATAAACGTG

VZV

VZV-F

TTGTGTCGGTCTCTCCAAGC

(934 bp)

VZV-R

TACGTCTTCAACCTCACGCC

CMV

CMV-F

TGGCTTTTCTTGAACGTGCG

(716 bp)

CMV-R

CCTTGACGCTGGTTTGGTTG

  1. F forward primer, R reverse primer, P probe, FAM 6-carboxy fluorescein, TAMRA 6-carboxy tetramethyl rhodamine, bp base pair