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Fig. 6 | Virology Journal

Fig. 6

From: Cohesin promotes HSV-1 lytic transcription by facilitating the binding of RNA Pol II on viral genes

Fig. 6

Cohesin components knockdown led to an overall decrease of RNA pol II binding and an increase of H3K27me3 binding to the HSV-1 genome. HeLa cells were transfected with control siRNA or siRNA targeting SMC1 or Rad21 for 48 h. Then cells were infected with HSV-1 at an MOI 5. The occupancy of RNA Polymerase II (a), RNA Polymerase II Phosphorylated on Serine 5 (b), and H3K27me3 (c) on both HSV-1 gene promoters (-P) and bodies (-B) were detected by ChIP-qPCR. Error bars represent the standard deviation from three independent experiments

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