Fig. 6
From: Cohesin promotes HSV-1 lytic transcription by facilitating the binding of RNA Pol II on viral genes
Cohesin components knockdown led to an overall decrease of RNA pol II binding and an increase of H3K27me3 binding to the HSV-1 genome. HeLa cells were transfected with control siRNA or siRNA targeting SMC1 or Rad21 for 48Â h. Then cells were infected with HSV-1 at an MOI 5. The occupancy of RNA Polymerase II (a), RNA Polymerase II Phosphorylated on Serine 5 (b), and H3K27me3 (c) on both HSV-1 gene promoters (-P) and bodies (-B) were detected by ChIP-qPCR. Error bars represent the standard deviation from three independent experiments