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Fig. 5 | Virology Journal

Fig. 5

From: Inhibition of Japanese encephalitis virus proliferation by long non-coding RNA SUSAJ1 in PK-15 cells

Fig. 5

Chromatin immunoprecipitation (CHIP) assay of transcription factor SP1 in the lncRNA-SUSAJ1 promoter region. (a–e) CHIP was performed using digested chromatin from CCR1 knockdown or scrambled siRNA treatment groups of PK-15 cells. Following immunoprecipitation with an anti-SP1 antibody, enrichment of the SP1-containing DNA sequence was quantified by quantitative RT-PCR. In (a–e), amounts of the SP1-containing DNA sequence are expressed relative to the lncRNA-SUSAJ1 input of each group (n = 3 per group). Histone H3 rabbit antibody and normal rabbit IgG were used as positive and negative controls, respectively. *P < 0.05 and **P < 0.01 VS si-scr

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