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Fig. 4 | Virology Journal

Fig. 4

From: Inhibition of Japanese encephalitis virus proliferation by long non-coding RNA SUSAJ1 in PK-15 cells

Fig. 4

Effects of CCR1 knockdown on the expression of transcription factor SP1. (a) mRNA levels of CEBPA, SP1, NF1, and GATA1 after transfection with CCR1 siRNA for 48 h, as determined by RT-PCR (n = 3 per group) *P < 0.05 VS si-scr. (b) Whole-cell lysates were harvested from the treatment groups as indicated. Immunoblotting was performed to detect SP1 protein levels using β-actin as an internal control. (c) Knockdown of SP1 in PK-15 cells by siRNAs. Cells were transfected with SP1 siRNAs or scrambled siRNA (n = 3 per group), *P < 0.05 VS si-scr. (d) Effects of SP1 and lncRNA-SUSAJ1 transcript levels were determined by RT-PCR after transfected with pcDNA3.1-SP1 and pcDNA3.1-NC (n = 3 per group), *P < 0.05 VS pcDNA3.1-NC

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