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Fig. 1 | Virology Journal

Fig. 1

From: Inhibition of Japanese encephalitis virus proliferation by long non-coding RNA SUSAJ1 in PK-15 cells

Fig. 1

Effects of lncRNA-SUSAJ1 overexpression on JEV proliferation. (a) Time course of lncRNA-SUSAJ1 transcript levels after JEV infection (n = 3 per group), *P < 0.05 and **P < 0.01 VS 0 h. (b) Effects of lncRNA-SUSAJ1 transcript levels were determined by reverse-transcription polymerase chain reaction (RT-PCR) after transfected with pcDNA3.1-lncRNA SUSAJ1 and NC (n = 3 per group), **P < 0.01 VS mock. (c) Whole-cell lysates were harvested from the treatment groups as indicated. Immunoblotting was performed to detect NS3 protein levels using β-actin as an internal control. (d) Effects of JEV transcript levels were determined by RT-PCR after transfected with pcDNA3.1-lncRNA SUSAJ1 and pcDNA3.1-NC (n = 3 per group), **P < 0.01 VS pcDNA3.1-NC

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