Skip to main content

Table 2 The 50% inhibitory concentration of infection (IC50) of St19pv and VSVpv by serum or whole blood from COVID-19 hospitalized patients

From: Evaluation of SARS-CoV-2 neutralizing antibodies using a vesicular stomatitis virus possessing SARS-CoV-2 spike protein

No

St19pv

VSVpv

Serum (IC50)

#6

 × 1600

 × 25

#7

 >  × 6400

 × 50

#12

 × 1600

 × 25

#20

 × 3200

 <  × 25

#21

 × 400

 <  × 25

#22

 × 200

 <  × 25

#23

 × 400

 <  × 25

Whole blood (IC50)

#6

 × 1600

 × 100

#7

 >  × 6400

 × 100

#12

 × 1600

 × 100

#20

 × 1600

 × 100

#21

 × 800

 × 100

#22

 × 400

 × 100

#23

 × 400

 × 100

  1. The pseudotyped viruses, St19pv or VSVpv, were preincubated with serial dilutions (× 25, × 50, × 100, × 200, × 400, × 800, × 1600, × 3200, × 6400) of serum or whole blood from seven different COVID-19 hospitalized patients (patients #6, #7, #12, #20, #21, #22, and #23) with culture media. Thereafter, Vero cells were infected with pseudotyped viruses. Infectivities of pseudotyped viruses were determined by measuring luciferase activities 24 h post-infection. IC50 value was calculated and determined as shown in the dilution range. The results are from three independent assays with error bars representing standard deviations