Table 2 Nanopore sequencing metrics for DENV RNA standards, using the multiplex (400 bp amplicon) approach
From: Assessment of a multiplex PCR and Nanopore-based method for dengue virus sequencing in Indonesia
Serotype | Genotype | Reads passing QC | Coding-Region Coverage (%) | Nucleotide Consensus Similarity (%) | ||
|---|---|---|---|---|---|---|
1X | 20X | 1X | 20X | |||
1 | I | 8604 | 100 | 96.51 | 99.92 | 99.88 |
2 | IV | 14,265 | 100 | 87.56 | 99.50 | 99.81 |
3 | V | 8337 | 100 | 89.93 | 99.28 | 99.69 |
4 | I | 16,654 | 100 | 89.96 | 99.25 | 99.72 |