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Fig. 2 | Virology Journal

Fig. 2

From: Assessment of a multiplex PCR and Nanopore-based method for dengue virus sequencing in Indonesia

Fig. 2

Nanopore Sequencing Coverage of Indonesian Clinical Isolates using the Multiplex PCR Approach. The multiplex PCR approach was used to amplify DENV1–4 from 10 clinical samples from Indonesia. These samples were selected to cover a range of viral loads, as estimated by Ct values from the diagnostic qRT-PCR. The resulting amplicons were sequenced on the Nanopore MinION. Coverage depth for each sample is plotted, with the read depth threshold of 20X indicated by the dotted line. Coverage depth is capped at 1000X

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