Fig. 1

Nanopore sequencing coverage for DENV control RNA samples, using the multiplex (400 bp amplicon) approach. Nanopore sequencing coverage depth for dengue virus control RNA samples, using the multiplex PCR approach is plotted in black against the left-hand y-axis, with the read depth threshold of 20X indicated by the dotted line. Coverage depth is capped at 1000X. The nucleotide similarity of the Nanopore-generated sequence to the Illumina-generated reference sequence is shown in red against the right-hand y-axis. The shaded areas identify regions where the coverage depth fell below the low coverage threshold of 20X, to allow comparison with the similarity plot