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Table 1 Primers used for PCR amplification of target genes in this study

From: Characterization of a recombinant pseudorabies virus expressing porcine parvovirus VP2 protein and porcine IL-6

Target genePrimerSequence a(5′-3′)Primer locationbRestriction siteExpected product (bp)
Porcine IL-6IL6fsGACGAATTCATGAACTCCCTCTCCACA1–18EcoR I639
IL6rsGTCGTTAAC CTACATTATCCGAATG471–488Hpa I 
PPV VP2VP2fsACCATGGGGGGGGTTGGTG2739–2758 1635
VP2rsATCCTATTTCTAGTATAATTT4359–4373  
PRV gHgH fsGCGTGTACTGCGACTGCGTGTT62,700–62,721 355
gH rsCGACCTGGCGTTTATTAACCGAGA63,032–63,055  
PPV NS1NS1fsCCAAAAATGCAAACCCCAATA1808–1828 194
NS1rsTCTGGCGGTGTTGGAGTTAAG1981–2001  
  1. aThe restriction enzyme sites used for the construction are underlined
  2. bThe primers IL6fs/IL6rs were designed to amplify porcine IL-6 gene using plasmid pIRES-IL6 as templates. The primers VP2fs/VP2rs and NS1fs/NS1rs were designed based on the nucleotide sequence (KF429255) of PPV HN genome to amplify VP2 and NS1genes, respectively. The primers gH fs/gH rs were designed to amplify gH gene based on PRV gH nucleotide sequence (KP257591) retrieved from the GenBank