Characterization of a recombinant pseudorabies virus expressing porcine parvovirus VP2 protein and porcine IL-6

Table 1 Primers used for PCR amplification of target genes in this study

Target gene	Primer	Sequence ^a(5′-3′)	Primer location^b	Restriction site	Expected product (bp)
Porcine IL-6	IL6fs	GACGAATTCATGAACTCCCTCTCCACA	1–18	EcoR I	639
Porcine IL-6	IL6rs	GTCGTTAAC CTACATTATCCGAATG	471–488	Hpa I
PPV VP2	VP2fs	ACCATGGGGGGGGTTGGTG	2739–2758		1635
PPV VP2	VP2rs	ATCCTATTTCTAGTATAATTT	4359–4373
PRV gH	gH fs	GCGTGTACTGCGACTGCGTGTT	62,700–62,721		355
PRV gH	gH rs	CGACCTGGCGTTTATTAACCGAGA	63,032–63,055
PPV NS1	NS1fs	CCAAAAATGCAAACCCCAATA	1808–1828		194
PPV NS1	NS1rs	TCTGGCGGTGTTGGAGTTAAG	1981–2001

^aThe restriction enzyme sites used for the construction are underlined
^bThe primers IL6fs/IL6rs were designed to amplify porcine IL-6 gene using plasmid pIRES-IL6 as templates. The primers VP2fs/VP2rs and NS1fs/NS1rs were designed based on the nucleotide sequence (KF429255) of PPV HN genome to amplify VP2 and NS1genes, respectively. The primers gH fs/gH rs were designed to amplify gH gene based on PRV gH nucleotide sequence (KP257591) retrieved from the GenBank

ISSN: 1743-422X