Fig. 1

Flowchart of the recombinant pseudorabies virus rPRV-VP2-IL6 expressing porcine parvovirus VP2 protein and porcine IL-6. The recombinant transfer plasmid pGVP2-IL6 was constructed and then transfected into the rPRV-VP2-EGFP-infected ST cells to generate the recombinant virus rPRV-VP2-IL6. a The porcine IL-6 digested by EcoR I and Hpa I. b PRV transfer plasmid pGVP2-EGFP containing an SphI–KpnI fragment of the unique short region of PRV strain Fa, SV40 Poly (A), an enhanced GFP (EGFP) expression cassette and the VP2 gene of PPV. c The PRV transfer plasmid pGVP2-IL6. The porcine IL-6 was cloned into EcoR I- and Hpa I-digested the plasmid pGVP2-EGFP to produce plasmid pGVP2-IL6. d The genome of rPRV-VP2-EGFP containing the DNA of parental PRV HB98, the SphI–KpnI fragment of the unique short region of PRV strain Fa, SV40 Poly (A), an EGFP expression cassette and the VP2 gene of PPV. e The genome of the recombinant virus rPRV-VP2-IL6. The plasmid pGVP2-EGFP was transfected into the rPRV-VP2-EGFP-infected ST cells to generate rPRV-VP2-IL6 containing SphI–KpnI fragment of the unique short region of PRV strain Fa, SV40 Poly (A), pig IL-6 and the VP2 gene of PPV