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Fig. 2 | Virology Journal

Fig. 2

From: Design and synthesis of HLA-A*02-restricted Hantaan virus multiple-antigenic peptide for CD8+ T cells

Fig. 2

Comparison of IFN-γ-secreting CD8+ T cell frequency and activity after HTNV MAP and single-peptide stimulation. IFN-γ-secreting cell frequency was detected by enzyme-linked immunospot (ELISPOT) assay. CD4+ T cell-depleted PBMCs from HLA-A*02+ patients with HFRS were stimulated with single peptide (80 μmol/L), MAP (80 μmol/L) or phytohemagglutinin (10 μg/mL). HTNV single CTL epitope LIWTGMIDL, VMASLVWPV, SLTECPTFL, and FVVPILLKA are represented by LL9, VV9, SL9, and FA9, respectively. a The frequency of IFN-γ-secreting CD8+ T cells was detected. All cell frequencies were converted to the number of spot-forming cells per 1 × 106 CD4+ T cell-depleted PBMCs (SFCs/106 cells). b The activity of IFN-γ-secreting CD8+ T cells was measured. The activity was evaluated as the average of the spot size and intensity for each well. Each black dot represents one patient sample. The Mann-Whitney U test was used to determine the difference between two groups. The black line indicates the median and corresponding interquartile range (IQR). *P < 0.05, **P < 0.01, ***P < 0.001, ns indicates no significant difference

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