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Table 2 Infectious titre production of ABBV-1 in immortalized cell lines

From: Isolation of Ontario aquatic bird bornavirus 1 and characterization of its replication in immortalized avian cell lines

Experiment 1

Homologous Titration (Log FFU / 106 cells)

Heterologous Titration (Log FFU / 106 cells)

Passage

CCL-141 ABBV-1

QT-35 ABBV-1

DF-1 ABBV-1

P value

CCL-141 ABBV-1

QT-35 ABBV-1

DF-1 ABBV-1

P value

8

5.11

2.68

NT

 

4.86

2.02

NT

 

12

3.98

2.43

NT

 

4.48

1.93

NT

 

13

3.92

3.18

NT

 

4.67

3.09

NT

 

Mean ± SD

4.34 ± 0.67

2.76 ± 0.38

N/A

*

4.67 ± 0.19

2.35 ± 0.65

N/A

**

Experiment 2

Homologous Titration (Log FFU / 106 cells)

 

Heterologous Titration (Log FFU / 106 cells)

 

Passage

CCL-141 ABBV-1

QT-35 ABBV-1

DF-1 ABBV-1

P value

CCL-141 ABBV-1

QT-35 ABBV-1

DF-1 ABBV-1

P value

6

4.57

1.82

NT

 

4.49

NT

NT

 

7

4.40

NT

NT

 

4.49

NT

NT

 

8

4.49

1.82

NT

 

4.66

NT

NT

 

10

3.32

2.90

NT

 

3.11

2.49

NT

 

11

3.61

2.66

NT

 

3.36

2.40

NT

 

12

2.94

2.74

NT

 

3.11

2.66

2.06

 

Mean ± SD

3.89 ± 0.69

2.39 ± 0.52

N/A

**

3.87 ± 0.75

2.52 ± 0.13

N/A

*

  1. NT – no measurable titre and not included in statistical analysis
  2. N/A – not applicable
  3. The mean is the average of all measurable titre within each experiment for each cell line. The SD is the standard deviation of all measurable titre within each experiment for each cell line. Mean ± SD was not calculated for DF-1 ABBV-1 because there was either no measurable titre or only one measurable titre
  4. * P < 0.05, ** P < 0.01. P value indicates statistical significance between CCL-141 ABBV-1 and QT-35 ABBV-1. Statistical analysis was not performed on DF-1 ABBV-1 due to lack of measurable titre for all but one passage
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Virology Journal

ISSN: 1743-422X