Fig. 3

CREB5 was a direct-targeted gene of miR-876-5p. a Predicted binding sites between miR-876-5p and 3’UTR of CREB5. b Luciferase activities of SF268 cells were determined through the luciferase reporter assay by cotransfection with CREB5–3’UTR or CREB5–3’UTR-mutant and miR-876-5p or miR-control. The expression level of CREB5 at mRNA (c) and protein (d) levels was measured through real-time quantitative polymerase chain reaction and Western blotting in SF268 cells transfected with miR-876-5p (lane 2), anti-miR-876-5p inhibitor (20 and 40 nM; lanes 3 and 4), or corresponding controls (lane 1). All experiments were repeated three times. * P < .05; ** P < .01