Fig. 1

miR-876-5p facilitated the EV-A71 infection cycle in human neuroblastoma SF268 cells. a SF268 cells were infected with EV-A71 at an MOI of 1, and the cells were harvested at different time points after infection, as indicated. The RNA was the isolation and expression level of miR-876-5p, which was determined through real-time quantitative polymerase chain reaction (RT-qPCR); b The expression level of miR-876-5p was detected through RT-qPCR after in the pmiR-876-5p transfected cells; c SF268 cells were expressed with miR-876-5p (lanes 2–3), followed by infection with EV-A71. The intracellular EV-A71 viral proteins, 3D/3CD, and VP1–3 were detected through Western blotting (lane 1: control cells; lanes 2–3: miR-876-5p overexpressed; lane 4: mock-infected cells). β-actin was loaded as the internal control. All experiments were repeated three times. ** P < .01