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Fig. 4 | Virology Journal

Fig. 4

From: Identification of a potential neutralizing linear epitope of hemagglutinin-neuraminidase in Newcastle disease virus

Fig. 4

Virus neutralization of the IDE-specific antisera. The IDE mouse antisera were serially diluted in 96-well plates and incubated with LaSota at 200 TCID50 (a), JS17 at 50 TCID50 (b) and F48E9 at 10 TCID50 (c). a Virus-neutralizing activities against LaSota. Twelve hours after infection, the cells were detected by IFA with the anti-HN guinea pig pAb. The number of positive cells and the inhibition rate were calculated. Percentage of inhibition was determined as follows: 100%—percent of infected cells/percent of the infected cells in negative serum for each dilution. b Virus-neutralizing activities against JS17. The cells were detected by IFA with anti-NDV HN monoclonal antibody. The calculation method is the same as above. c Virus-neutralizing activities against F48E9. The number of syncytia was observed at different times after virus infection. Percentage of inhibition was determined as follows: 100%—the average number of syncytia/the average number of syncytia in negative serum for each dilution. Representative data of the IDEs and HN341–355 antisera compared to the negative serum, shown as the means ± SDs (n = 3), were analyzed by two-tailed Student’s t test. *P < 0.05; ***P < 0.001

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