Fig. 3

Immunogenicity of the IDE peptides. a The antibody titers of anti-IDE and anti-HN341-355 mouse sera by ELISAs. The nonimmunized mouse serum was used as a negative control. Representative data, shown as the means ± SDs (n = 3), were analyzed by two-tailed Student’s t test. ***P < 0.001. b Dot blot analysis. The IDE synthetic peptides (2 μg) were dropped on a nitrocellulose membrane and incubated with the IDE mouse antisera. KLH was used as a negative control. c The cross-reactivity of IDE mouse antisera by Western blot. RFP was used as a negative control. d The detection of RFP-IDEs by Western blot. The purified LaSota virus was used as a positive control. Single RFP expression was used as a negative control. e IDE mouse antisera recognizing LaSota virus. DF-1 cells were infected with LaSota at an MOI of 1. The cells were fixed at 24 h post-infection and analyzed by IFA with IDE mouse antisera