Fig. 2

Identification of IDEs. a IFA analysis of IDEs. BHK-21 cells were transfected with IDE-RFP constructs. After 48 h, the cells were fixed and incubated with HN-specific guinea pig pAb and subsequently incubated with an FITC-conjugated goat anti-mouse antibody. The cell nuclei were stained with 4′,6′-diamidino-2-phenylindole (DAPI). The cells were observed by fluorescence microscopy. Scale bar = 50 µm. RFP (red), IDEs and HN341-355 (green) as well as the cell nucleus (blue) are shown. b ELISA data of the IDEs. The 96-well plates were coated with 200 ng of each synthetic IDE and HN341-355 peptide and incubated with NDV-specific mouse antisera. The purified LaSota virus was used as a positive control. KLH was used as a negative control. **P < 0.01; ***P < 0.001