Fig.3

Strand transfer inhibition efficiency of catalytic and non-catalytic integrase inhibitors. a Schematic diagram of the strand transfer assay process. The yellow and blue boxes represent the response under conditions of no IN inhibitor or INSTI, respectively. b Integrase inhibitor compounds at a range of concentrations generated from fivefold serial dilutions were used to treat 3′-end cleaved LTR DS DNA by integrase. After incubation, TS DNA was added to each well for the strand transfer reaction. The reaction products were detected colorimetrically using a HRP-labeled antibody against modified target DNA. "No integrase" and "Integrase only" wells were set as the negative and positive control, respectively