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Fig. 2 | Virology Journal

Fig. 2

From: Validation of multiplex PCR sequencing assay of SIV

Fig. 2

a Number of false positives detected per nucleotide with coverage of at least 1800 and a variant frequency of at least 1% in at least one replicate per input copy (closed circle) or at least two replicates per input copy (open circle) for our Method 1 (vRNA) (left) and Method 2 (cDNA) (right) data sets. Lines represent median ± 95% confidence interval. No significant differences were identified between data sets by Kruskal–Wallis tests. b False positive variant frequency of variants identified in all input templates for Method 1 data sets. c False positive variant frequency of variants identified in all input templates for Method 2 data sets. cDNA input template numbers denoted by colors. Lines represent mean and standard deviation for each variant’s replicate. All variants shown are present at a frequency of 1% or greater, have a nucleotide depth of at least 1800, and are detected in at least two samples

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