Fig. 1From: Neutralization assay with SARS-CoV-1 and SARS-CoV-2 spike pseudotyped murine leukemia virionsInfectivity of pseudotyped MLV Viruses. SARS-CoV-2 spike pseudotyped MLV viruses as well as VSV-G, SARS-CoV-1 spike, and HIV-1 LAIgp160 pseudotyped MLV viruses were tested on HEK293FT, HEK293T-ACE2, Huh7 and SupT1 cells. 100 μL of undiluted virus (except for VSV-G pseudotype, which was diluted 1:100) was mixed with 100 μL of medium and added to cells. Luciferase was measured at 2 days post-infection and values are per well of a 96-well plate. Negative line indicates mean + 3SD of luciferase values obtained with virions devoid of glycoproteinBack to article page