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Table 2 Allplex™ 2019-nCoV assay interpretation

From: Efficient SARS-CoV-2 detection in unextracted oro-nasopharyngeal specimens by rRT-PCR with the Seegene Allplex™ 2019-nCoV assay

 

HEX (IC)

FAM (E gene)

Cal Red 610 (RdRP gene)

Quasar 670 (N gene)

Interpretation

Case 1

±

+

+

+

2019-nCoV detected

Case 2

±

+

+

2019-nCoV detected

Case 3

±

+

+

2019-nCoV detected

Case 4

±

+

+

2019-nCoV detected

Case 5

±

+

2019-nCoV detected

Case 6

±

+

2019-nCoV detected

Case 7

±

+

Presumptive positive

Case 8

+

Negative

Case 9

Invalid

  1. Ct ≤ 40 = detected (+); Ct > 40 = non detected (−); Ct IC > 40 = invalid (−)