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Fig. 2 | Virology Journal

Fig. 2

From: Efficient SARS-CoV-2 detection in unextracted oro-nasopharyngeal specimens by rRT-PCR with the Seegene Allplex™ 2019-nCoV assay

Fig. 2

Proteinase K improves efficiency and accuracy of detection. 90 known positive RNase-free water samples for three viral genes (a, b), 60 known RNase-free water positive samples for N gene only (c, d), and 60 known RNase-free water negative samples (e, f) were divided in 3 equal groups and analysed in 3 independent experiments by rRT-PCR on E, RdRP and N viral genes with dilution 1/2 and thermal lysis treatment and with or without a PK treatment at 100 µg/ml. Red numbers indicate the percentage of detected samples. a, c, e Comparison of Ct values of viral genes. b, d, f Distribution of invalid, negative, or positive results. Dots represent the mean of three random groups of 30 samples each (Set 3) or 20 samples each (set 4 and 5)

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