Skip to main content

Table 5 The results of qRT-PCR, OSN-qRT-PCR and ddPCR and further clinical information of 34 clinical samples from 24 acute phase COVID-19 patients

From: Analysis and validation of a highly sensitive one-step nested quantitative real-time polymerase chain reaction assay for specific detection of severe acute respiratory syndrome coronavirus 2

Sample ID

Results of qRT-PCR (Ct Value)

Results of OSN-qRT-PCR(Ct Value)

Results of ddPCR (copies/ml)

Specimen type

Initial official reports by OSN-qRT-PCR

Judgment by N-PCR

Judgment by ddPCR

ORF1ab

N

ORF1ab

N

ORF1ab

N

1

27.20

26.94

15.00

16.68

129,415

1287.00

Throat swab

P

P

P

2

35.04

35.35

22.60

22.80

428.90

572.00

Throat swab

P

P

P

3

37.11

37.22

Throat swab

N

N

N

4

34.46

34.95

21.74

22.76

500.50

715.00

Throat swab

P

P

P

5

31.31

30.66

17.39

19.21

5148.00

10,224.50

Throat swab

P

P

P

6

28.02

27.38

14.65

15.25

74,360.0

364.45

Throat swab

P

P

P

7

38.21

Throat swab

N

N

N

8

32.61

32.70

18.17

19.96

2860.00

3503.50

Throat swab

P

P

P

9a

40.32

23.50

24.64

228.80

429.00

Throat swab

N

P

S

10

35.50

35.10

23.40

23.96

715.00

715.00

Throat swab

P

P

P

11

35.08

33.09

23.60

22.14

715.00

2145.00

Throat swab

P

P

P

12

26.58

26.76

12.52

13.95

215,930

4336.0

Throat swab

P

P

P

13a

40.83

28.75

26.19

157.30

228.80

Throat swab

N

P

N

14

34.00

33.96

20.84

23.19

1930.50

1716.00

Throat swab

P

P

P

15

34.37

34.38

21.70

23.10

2216.50

1144.00

Throat swab

P

P

P

16

38.56

Throat swab

N

N

N

17

34.33

34.27

22.08

20.28

1215.50

2502.50

Throat swab

P

P

P

18a

40.21

28.67

26.88

300.30

786.50

Throat swab

N

P

S

19

29.29

29.42

18.21

18.77

31,460

42,900

Sputum

P

P

P

20

36.11

572.00

1787.50

Sputum

N

N

P

21

29.89

29.25

18.30

19.36

13,799

30,530

Sputum

P

P

P

22

30.45

29.82

21.82

23.99

Sputum

P

P

N

23

26.79

26.94

15.78

17.50

23,595.0

14,657.50

Sputum

P

P

P

24a

40.81

35.98

36.21

1430.0

sputum

N

N

N

25a

40.21

27.88

25.63

643.50

1215.50

Sputum

N

P

P

26

31.10

30.32

22.56

21.94

9509.50

3789.50

Sputum

P

P

P

27

27.85

27.45

20.67

20.34

84,370.0

170,170.0

Sputum

P

P

P

28

29.89

28.22

22.10

21.31

643.50

Sputum

P

P

N

29a

40.22

16.62

17.08

715.0

715.0

Blood

N

P

P

30

37.90

Blood

N

N

N

31

35.08

34.73

21.57

22.83

500.50

715.00

Anal swab

P

P

P

32a

40.55

25.20

24.68

214.50

Anal swab

N

P

N

33a

40.06

24.00

25.68

250.25

Anal swab

N

P

N

34

26.66

27.22

307.45

Anal swab

N

P

N

  1. aOne or two of the target genes had a Ct value happen to fall in the gray zone (Ct value: 37–40), and samples were retested by qRT-PCR. At last, these samples were considered as negative with Ct value > 40 (both targets genes) by qRT-PCR
  2. P, positive; N, Negative; S, suspect; GGO, ground glass opacities image