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Table 5 The results of qRT-PCR, OSN-qRT-PCR and ddPCR and further clinical information of 34 clinical samples from 24 acute phase COVID-19 patients

From: Analysis and validation of a highly sensitive one-step nested quantitative real-time polymerase chain reaction assay for specific detection of severe acute respiratory syndrome coronavirus 2

Sample ID Results of qRT-PCR (Ct Value) Results of OSN-qRT-PCR(Ct Value) Results of ddPCR (copies/ml) Specimen type Initial official reports by OSN-qRT-PCR Judgment by N-PCR Judgment by ddPCR
ORF1ab N ORF1ab N ORF1ab N
1 27.20 26.94 15.00 16.68 129,415 1287.00 Throat swab P P P
2 35.04 35.35 22.60 22.80 428.90 572.00 Throat swab P P P
3 37.11 37.22 Throat swab N N N
4 34.46 34.95 21.74 22.76 500.50 715.00 Throat swab P P P
5 31.31 30.66 17.39 19.21 5148.00 10,224.50 Throat swab P P P
6 28.02 27.38 14.65 15.25 74,360.0 364.45 Throat swab P P P
7 38.21 Throat swab N N N
8 32.61 32.70 18.17 19.96 2860.00 3503.50 Throat swab P P P
9a 40.32 23.50 24.64 228.80 429.00 Throat swab N P S
10 35.50 35.10 23.40 23.96 715.00 715.00 Throat swab P P P
11 35.08 33.09 23.60 22.14 715.00 2145.00 Throat swab P P P
12 26.58 26.76 12.52 13.95 215,930 4336.0 Throat swab P P P
13a 40.83 28.75 26.19 157.30 228.80 Throat swab N P N
14 34.00 33.96 20.84 23.19 1930.50 1716.00 Throat swab P P P
15 34.37 34.38 21.70 23.10 2216.50 1144.00 Throat swab P P P
16 38.56 Throat swab N N N
17 34.33 34.27 22.08 20.28 1215.50 2502.50 Throat swab P P P
18a 40.21 28.67 26.88 300.30 786.50 Throat swab N P S
19 29.29 29.42 18.21 18.77 31,460 42,900 Sputum P P P
20 36.11 572.00 1787.50 Sputum N N P
21 29.89 29.25 18.30 19.36 13,799 30,530 Sputum P P P
22 30.45 29.82 21.82 23.99 Sputum P P N
23 26.79 26.94 15.78 17.50 23,595.0 14,657.50 Sputum P P P
24a 40.81 35.98 36.21 1430.0 sputum N N N
25a 40.21 27.88 25.63 643.50 1215.50 Sputum N P P
26 31.10 30.32 22.56 21.94 9509.50 3789.50 Sputum P P P
27 27.85 27.45 20.67 20.34 84,370.0 170,170.0 Sputum P P P
28 29.89 28.22 22.10 21.31 643.50 Sputum P P N
29a 40.22 16.62 17.08 715.0 715.0 Blood N P P
30 37.90 Blood N N N
31 35.08 34.73 21.57 22.83 500.50 715.00 Anal swab P P P
32a 40.55 25.20 24.68 214.50 Anal swab N P N
33a 40.06 24.00 25.68 250.25 Anal swab N P N
34 26.66 27.22 307.45 Anal swab N P N
  1. aOne or two of the target genes had a Ct value happen to fall in the gray zone (Ct value: 37–40), and samples were retested by qRT-PCR. At last, these samples were considered as negative with Ct value > 40 (both targets genes) by qRT-PCR
  2. P, positive; N, Negative; S, suspect; GGO, ground glass opacities image