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Table 3 Comparison of the repeatability (CV%) of the each assay

From: Analysis and validation of a highly sensitive one-step nested quantitative real-time polymerase chain reaction assay for specific detection of severe acute respiratory syndrome coronavirus 2

Method Conc. (copies/ml) ORF1ab N
SD Mean CV (%) SD Mean CV (%)
qRT-PCR 20,000 0.61 30.69 1.99 1.05 29.02 3.61
4000 1.36 33.22 4.09 1.81 31.92 5.67
2000 0.93 34.52 2.70 0.96 33.06 2.91
1000 1.55 35.76 4.32 2.02 34.07 5.92
500 0.58 36.30 1.60 1.86 36.00 5.16
200 0.84 37.99 2.20 1.69 37.08 4.56
OSN-qRT-PCR 20,000 0.49 13.87 3.57 0.09 19.21 0.47
4000 0.83 16.65 4.97 0.13 22.19 0.61
2000 1.35 18.85 7.15 0.38 23.68 1.62
1000 0.46 19.33 2.36 0.50 24.23 2.06
500 0.56 20.56 2.71 0.97 25.09 3.86
200 0.32 21.81 1.47 1.31 26.30 4.99
ddPCR 143,000 5644.4 107,327.3 5.26 2862.1 86,757.2 3.30
33,000 1999.9 28,569.7 7.00 1902.0 22,665.4 8.39
8000 458.7 9189.7 4.99 164.9 8094.9 2.04
2000 75.8 2085.0 3.63 340.8 4009.4 8.50
500 51.2 671.7 7.62 62.3 557.1 11.18
250 22.4 261.7 8.55 37.8 371.4 10.18
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