Table 3 Comparison of the repeatability (CV%) of the each assay
Method | Conc. (copies/ml) | ORF1ab | N | ||||
|---|---|---|---|---|---|---|---|
SD | Mean | CV (%) | SD | Mean | CV (%) | ||
qRT-PCR | 20,000 | 0.61 | 30.69 | 1.99 | 1.05 | 29.02 | 3.61 |
4000 | 1.36 | 33.22 | 4.09 | 1.81 | 31.92 | 5.67 | |
2000 | 0.93 | 34.52 | 2.70 | 0.96 | 33.06 | 2.91 | |
1000 | 1.55 | 35.76 | 4.32 | 2.02 | 34.07 | 5.92 | |
500 | 0.58 | 36.30 | 1.60 | 1.86 | 36.00 | 5.16 | |
200 | 0.84 | 37.99 | 2.20 | 1.69 | 37.08 | 4.56 | |
OSN-qRT-PCR | 20,000 | 0.49 | 13.87 | 3.57 | 0.09 | 19.21 | 0.47 |
4000 | 0.83 | 16.65 | 4.97 | 0.13 | 22.19 | 0.61 | |
2000 | 1.35 | 18.85 | 7.15 | 0.38 | 23.68 | 1.62 | |
1000 | 0.46 | 19.33 | 2.36 | 0.50 | 24.23 | 2.06 | |
500 | 0.56 | 20.56 | 2.71 | 0.97 | 25.09 | 3.86 | |
200 | 0.32 | 21.81 | 1.47 | 1.31 | 26.30 | 4.99 | |
ddPCR | 143,000 | 5644.4 | 107,327.3 | 5.26 | 2862.1 | 86,757.2 | 3.30 |
33,000 | 1999.9 | 28,569.7 | 7.00 | 1902.0 | 22,665.4 | 8.39 | |
8000 | 458.7 | 9189.7 | 4.99 | 164.9 | 8094.9 | 2.04 | |
2000 | 75.8 | 2085.0 | 3.63 | 340.8 | 4009.4 | 8.50 | |
500 | 51.2 | 671.7 | 7.62 | 62.3 | 557.1 | 11.18 | |
250 | 22.4 | 261.7 | 8.55 | 37.8 | 371.4 | 10.18 | |