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Table 2 Estimated limit of detection for SARS-COV-2 in copies/ml for each assay

From: Analysis and validation of a highly sensitive one-step nested quantitative real-time polymerase chain reaction assay for specific detection of severe acute respiratory syndrome coronavirus 2

Methods

LoD determined in assays (copies/ml)

ORFlab

N

qRT-PCR

520.1 (95% CI: 363.23–1145.69)

528.1 (95% CI: 347.7–1248.7)

OSN-qRT-PCR

194.74 (95% CI: 139.7–430.9)

189.1 (95% CI: 130.9–433.9)

ddPCR

401.8 (95% CI: 284.8–938.3)

336.8 (95% CI: 244.6–792.5)