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Fig. 2 | Virology Journal

Fig. 2

From: Analysis and validation of a highly sensitive one-step nested quantitative real-time polymerase chain reaction assay for specific detection of severe acute respiratory syndrome coronavirus 2

Fig. 2

The correlation analysis between OSN-qRT-PCR and ddPCR with qRT-PCR for quantifying SARS-CoV-2 pseudoviral RNA standards for ORF1ab and N gene. a, b the CT values of qRT-PCR of ORF1ab (a) and N gene (b) were plotted on the X axis versus the CT values of OSN-qRT-PCR of ORF1ab and N gene on the Y axis, the Person Correlation Coefficient between qRT-PCR and OSN-qRT-PCR for ORF1ab and N gene were 0.887 and 0.742. c, d The CT values of qRT-PCR of ORF1ab (c) and N gene (d) were plotted on the X axis versus the measured values(converted to log10) of ddPCR of ORF1ab and N gene on the Y axis, the Person Correlation Coefficient between qRT-PCR and ddPCR for ORF1ab and N gene were − 0.924 and − 0.844

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