Fig. 2

The broad-spectrum neutralization of influenza viruses against mAb 3C12. a Western Blot (WB) analysis. The lysates of influenza virus H1-H16-infected MDCK cells and H17 and H18 HA protein transfected 293 T cells were incubated with the mAb 3C12. b Indirect immunofluorescence assays (IFA). MDCK cells were infected with H1-H16 and flu B for no more than 24 h, 293 T cells were transfected with pCAGGS-H17 HA and H18 HA for 24 h, and then the cells were fixed and incubated with mAb 3C12. c IFA. MDCK cells were infected with other strains of subtype H1 and H9. NC served as negative control