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Table 1 The sgRNA sequences target PRV-Fa gE, gI, TK genes

From: The recombinant pseudorabies virus expressing African swine fever virus CD2v protein is safe and effective in mice

A. Primers name B. sgRNA sequence 5′–3′
PRV-TK-sgRNA-F 5′-caccgTGCCCGAGCCGATGGCGTGC-3′
PRV-TK-sgRNA-R 5′-aaacGCACGCCATCGGCTCGGGCAc-3′
PRV-gE-sgRNA-F 5′-caccgGCCGGCGACGATGACCTCGA-3′
PRV-gE-sgRNA-R 5′-aaacTCGAGGTCATCGTCGCCGGCc-3′
PRV-gI-sgRNA-F 5′-caccgCGCGGGGTCGTACGTGCTGC-3′
PRV-gI-sgRNA-R 5′-aaacGCAGCACGTACGACCCCGCGc-3′
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