Table 1 The sgRNA sequences target PRV-Fa gE, gI, TK genes
A. Primers name | B. sgRNA sequence 5′–3′ |
|---|---|
PRV-TK-sgRNA-F | 5′-caccgTGCCCGAGCCGATGGCGTGC-3′ |
PRV-TK-sgRNA-R | 5′-aaacGCACGCCATCGGCTCGGGCAc-3′ |
PRV-gE-sgRNA-F | 5′-caccgGCCGGCGACGATGACCTCGA-3′ |
PRV-gE-sgRNA-R | 5′-aaacTCGAGGTCATCGTCGCCGGCc-3′ |
PRV-gI-sgRNA-F | 5′-caccgCGCGGGGTCGTACGTGCTGC-3′ |
PRV-gI-sgRNA-R | 5′-aaacGCAGCACGTACGACCCCGCGc-3′ |