Fig. 8
After immunization, the recombinant attenuated virus can produce anti-CD2v specific antibody and specific cell response and has a 100% protective effect against challenge by virulent strains. a Anti-CD2v specific antibody can be produced by immunizing the recombinant strain. Five-week-old SPF-ICR mice were immunized with 1 × 105 TCID50 viruses by intramuscular injection in the right hind leg. The second immunization was carried out 7 days after the first immunization. The control group was injected with 100 μL DMEM. About 200 μl blood was collected from the orbit before and 7 days after each immunization for ELSIA to detect Flag antibody (n = 5/each group). b IFNγ was detected at high transcription level when PBMC was stimulated with purified (N-CD2v)-His (n = 5/each group). c Recombinant strain can 100% protect mice from virulent virus challenge. After the second immunization for 7 days, challenged with 5 × 105 TCID50 PRV-Fa, and the survival curve was drawn (n = 15/each group). d There was less detoxification in the recombinant attenuated virus-infected groups in response to the PRV-Fa challenge. After the PRV-Fa challenge, the feces of mice were collected every day to detect the number of virus copies (n = 5/each group). Two-way ANOVA was performed by GraphPad Prism 5.0, GraphPad Software (San Diego, CA, USA), ***p < 0.001