Fig. 7
PRV-Fa, PRV-ΔgE/ΔgI/ΔTK and PRV-ΔgE/ΔgI/ΔTK-(CD2v) inoculation led to T cells' activation. a–d The result of CD3+CD69+ T cell flow analysis. f–i: The result of CD4+CD69+ T cell flow analysis. k–n: The result of CD8+CD69+ T cell flow analysis. The data showed the changes of the percentage of CD3+CD69+ (e), CD4+CD69+ (j), CD8+CD69+ (o) T cells in total PBMCs. Five-week-old SPF-ICR mice were inoculated with 1 × 105 TCID50 viruses by intramuscular injection in the right hind leg. The control group was injected with 100 μL DMEM. When the PRV-Fa-infected group started to scratch (about 72 hpi), the target cells were collected and analyzed by flow cytometry. Unpaired t-test was performed by GraphPad Prism 5.0, GraphPad Software (San Diego, CA, USA), *p < 0.05, **p < 0.01, ***p < 0.001, (n = 5/each group)