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Fig. 3 | Virology Journal

Fig. 3

From: The recombinant pseudorabies virus expressing African swine fever virus CD2v protein is safe and effective in mice

Fig. 3

The virus DNA copies present in the various organs of mice inoculation by viruses, including heart (a), liver (b), spleen (c), lung (e), kidney (f), and brain (g) at 72 hpi. The virus DNA copies present in the various organs of mice inoculation by viruses, including the brain (d) and lung (h) at 200 hpi. Five-week-old SPF-ICR mice were inoculated with 1 × 105 TCID50 viruses by intramuscular injection in the right hind leg. The control group was injected with 100 μL DMEM. When the PRV-Fa infected group started to scratch (about 72 hpi) or extended the infection time to 200 h, the mice of different viral treated and untreated groups were sacrificed, and the organs were collected for genomic DNA extraction, and 100 ng genome was used to perform qPCR analyses to determine viral nucleic acid copy number (n = 5/each group), ND (not detect)

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