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Table 1 Primers and reaction cycling conditions for pol and env

From: Characteristics of HIV-1 molecular transmission networks and drug resistance among men who have sex with men in Tianjin, China (2014–2018)

Gene region Primers Reaction cycling conditions
env
  ED5 5′-ATGGGATCAAAGCCTAAAGCCATGTG-3′ (sense) RT-PCR:
50 °C 30 min 94 °C 2 min; 30 cycles of 94 °C 30 s, 55 °C 30 s, 72 °C 80 s; 72 °C 10 min
  ED12 5′-AGTGCTTCCTGCTGCTCCCA-3′ (anti-sense)  
  Env7a 5′-CTGTTAAATGGCAGTCTAGC-3′ (sense) The second round:
94 °C 3 min, 30 cycles of 94 °C 30 s, 55 °C 30 s, 72 °C 80 s; 72 °C 10 min
  Env7a 5′-CTGTTAAATGGCAGTCTAGC-3′ (anti-sense)  
pol
  MAW-26 5′-TGGAAATGTGGAAAGGAAGGAC-3′ (sense) RT-PCR:
50 °C 30 min, 94 °C 2 min; 30 cycles of 94 °C 30 s, 55 °C 30 s, 72 °C 150 s; 72 °C 10 min
  RT-21 5′-CTGTATTTCTGCTATTAAGTCTTTTGA-3′ (anti-sense)  
  PRO-1a 5′-CAGAGCCAACAGCCCCACCA-3′ (sense) The second round:
94 °C 5 min; 30 cycles of 94 °C 30 s, 63 °C 30 s, 72 °C 150 s; 72 °C 10 min
  RT-20a 5′-CTGCCAGTTCTAGCTCTGCTTC-3′ (anti-sense)  
  RT4Ra 5′-CTTCTGTATATCATTGACAGTCCAGCT-3′ (anti-sense) Sequencing
  RT1a 5′-CCAAAAGTTAAACAATGGCCATTGACAGA-3′ (sense) Sequencing
  PROC1a 5′-GCTGGGTGTGGTATTCC-3′ (anti-sense) Sequencing
  1. aSequencing primers